Posts Tagged ‘Protein’
Last Updated on Sunday, 18 July 2010 07:44 Written by Administrator Sunday, 18 July 2010 07:44
Protein Blotting: A Practical Approach (The Practical Approach Series 140)
Protein blotting techniques have become common laboratory procedures in the past few years. This text is written by scientists with expertise in these techniques. The versatility of the methods utilizing these procedures have brought about the development of different solid support matrices, as well as a wide variety of protein detection methods. While the most commonly used method in protein blotting is the use of antibodies to detect protein antigens, this technology has been expanded to examine a number of different interactions between proteins and other proteins, as well as other molecules such as carbohydrates and DNA. These methods have further been adapted for amino acid sequencing and purification of proteins for use as immunogens. This book outlines, in detail, numerous protocols and procedures which should help investigators design methods which will be optimal for their specific use.
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Tags: Approach, Blotting, Practical, Protein, Series | Posted under Western Blot Books | No Comments
Last Updated on Sunday, 18 July 2010 07:44 Written by Administrator Sunday, 18 July 2010 07:44
Protein Blotting and Detection: Methods and Protocols (Methods in Molecular Biology)
Over the past thirty years, the development of the Western blot has revolutionized the fields of biomedical research and medical diagnostics. In “Protein Blotting and Detection: Methods and Protocols”, expert researchers present numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting. Chapters offer a large number of variations on the theme of protein transfer to solid support followed by detection, presenting both adaptations of traditional techniques as well as completely original methods of protein blotting. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, a list of necessary materials, step-by-step methods, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
Innovative and highly practical, Protein Blotting and Detection: Methods and Protocols is an essential, hands-on guide for all investigators who hope to bring these cutting-edge procedures home to their laboratories.
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Tags: Biology, Blotting, Detection, Methods, Molecular, Protein, Protocols | Posted under Western Blot Books | No Comments
Last Updated on Sunday, 18 July 2010 07:44 Written by Administrator Sunday, 18 July 2010 07:44
Protein Methods: Gel Electrophoresis, X-Ray Crystallography, Green Fluorescent Protein, Western Blot, Bimolecular Fluorescence Complementation
Purchase includes free access to book updates online and a free trial membership in the publisher’s book club where you can select from more than a million books without charge. Chapters: Gel Electrophoresis, X-Ray Crystallography, Green Fluorescent Protein, Western Blot, Bimolecular Fluorescence Complementation, Structural Alignment, Homology Modeling, Protein Nuclear Magnetic Resonance Spectroscopy, Immunoprecipitation, Protein Purification, Methylated Dna Immunoprecipitation, Chip-On-Chip, Protein Structure Prediction, Enzyme Assay, Immunohistochemistry, Secondary Structure Prediction, Eastern Blotting, Macromolecular Crowding, Protein Sequencing, Chromatin Immunoprecipitation, Threading, Phi Value Analysis, Protein Subcellular Localization Prediction, Dead-End Elimination, Immunostaining, Kaede, List of Protein Structure Prediction Software, Peptide Mass Fingerprinting, Protein Microarray, Particle-Induced X-Ray Emission, Immunocytochemistry, Electrophoretic Mobility Shift Assay, Streptamer, Chip-Sequencing, Strep-Tag, Bradford Protein Assay, Immunoelectrophoresis, Aequorin, Self-Consistent Mean Field, Chevron Plot, Coomassie, Chou-Fasman Method, Kodecyte, Native Page, Columnmaster 4000, Activity Based Proteomics, Magnet Assisted Transfection, Photoactivatable Fluorescent Protein, Biuret Test, Loop Modeling, de Novo Protein Structure Prediction, Electroblotting, Itraq, Protein Electrophoresis, Expanded Bed Adsorption, Hnca Experiment, Hncoca Experiment, Gor Method, Lowry Protein Assay, Clip-Seq, Timestamp Protein Labelling, Synapto-Phluorin, Helical Wheel, Electrochromatography, Affinity Electrophoresis, Eos, Phosphoamino Acid Analysis, Cafasp, Rip-Chip, Help Conquer Cancer, Ponceau S. Excerpt: X-ray crystallography is a method of determining the arrangement of atoms within a crystal, in which a beam of X-rays strikes a crystal and diffracts into many specific directions. From the angles and intensities of these diffracted… More: http://booksllc.net/?id=34151
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Tags: Bimolecular, Blot, Complementation, Crystallography, Electrophoresis, Fluorescence, Fluorescent, Green, Methods, Protein, Western, XRay | Posted under Western Blot Books | No Comments