Posts Tagged ‘Comparative’
Last Updated on Sunday, 18 July 2010 07:45 Written by Administrator Sunday, 18 July 2010 07:45
DNA polymerase @b mRNA and protein expression in Xiphophorus fish [An article from: Comparative Biochemistry and Physiology, Part C]
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Herein we report Xiphophorus DNA polymerase @b (XiphPol@b) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase @b protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Pol@b expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis.
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Tags: article, Biochemistry, Comparative, expression, fish, from, mRNA, Part, Physiology, polymerase, Protein, Xiphophorus | Posted under Western Blot Books | No Comments
Last Updated on Sunday, 18 July 2010 07:44 Written by Administrator Sunday, 18 July 2010 07:44
Bacterial infection and tissue-specific Hsp72, -73 and -90 expression in western painted turtles [An article from: Comparative Biochemistry and Physiology, Part C]
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Heat shock proteins (Hsps) are molecular chaperones that assist intracellular folding, assembly and translocation of proteins in prokaryotic and eukaryotic cells. A variety of stresses including hyperthermia, radiation, heavy metals, ischemia, anoxia and reoxygenation have been shown to increase the expression of Hsps. Likewise, bacterial infection represents a stress for the host cell. In this study, expression of the constitutive (Hsp73) and inducible (Hsp72) isoforms of Hsp70 and Hsp90 was monitored in brain, heart, liver and skeletal muscle from the western painted turtle Chrysemys picta bellii diagnosed with Septicemic Cutaneous Ulcerative Dermatitis (SCUD). This disease is caused by a gram-negative bacterium probably belonging to the Citrobacter spp. The expression of Hsp73 increased 1.8-fold in brain and liver, 2.2-fold in heart but did not change in skeletal muscle; Hsp72 expression increased 5.5-fold in brain and 3-fold in liver but did not change in heart or skeletal muscle; Hsp90 expression increased 9-fold in brain, 2.7-fold in heart and 2.4-fold in skeletal muscle but did not change in liver. These results suggest a tissue-specific Hsp response during bacterial infection and a role for Hsps in immunopathological events in reptiles.
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Tags: article, Bacterial, Biochemistry, Comparative, expression, from, Hsp72, infection, painted, Part, Physiology, tissuespecific, turtles, Western | Posted under Western Blot Books | No Comments