Posts Tagged ‘Acta’
Last Updated on Thursday, 22 July 2010 03:23 Written by Administrator Thursday, 22 July 2010 03:23
Recombinant bovine somatotropin misuse in cattle [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Somatotropins (growth hormone, GH) which are used in cattle for growth and lactating performances are difficult to reliably detect since no direct method exists. Until now, the detection of GH in bovine milk, plasma and tissue has been based on radioimmunoassays and enzyme-linked immunosorbent assay (ELISA) tests that are unable to distinguish between endogenous and recombinant hormones. Attempt to directly detect recombinant GH administration is an analytical challenge which has never been successfully reported. The aim of the present work was to develop an original method, based on the detection in serum of antibodies raised against growth hormones as a consequence of their administration to animals. A second approach was developed with the characterisation of recombinant bovine somatotropin (rbST) in milk through the use of two-dimensional electrophoresis coupled to in-gel tryptic digestion followed by tandem mass spectrometry coupled to liquid chromatography (LC-ESI-MS/MS) measurements of the produced peptides. Results obtained showed the relevance of the two approaches. The Western blot one, carried out in this work on real biological blood samples collected on treated animals, would fit as a rapid and rather sensitive screening method, which can further more be achieved more over a long period after the administration of recombinant hormone. The second method, which consisted in a proteomic approach, dealt with the development of a confirmatory technique. Results on spiked milk samples were very promising and showed the need of further developments in this way.
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Tags: Acta, Analytica, article, bovine, cattle, Chimica, from, misuse, Recombinant, somatotropin | Posted under Western Blot Books | No Comments
Last Updated on Sunday, 18 July 2010 07:44 Written by Administrator Sunday, 18 July 2010 07:44
Western blot detection of brain material in heated meat products using myelin basic protein and neuron-specific enolase as biomarkers [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
An assay based on Western blotting and detection of central nervous system (CNS)-specific antigens was developed to detect brain tissue in processed (heated) meat products. Bands of antigen-bound primary antibodies were visualised through secondary anti-antibodies labelled with peroxidase, which generated chemiluminescence documented by a photographic film. Ponceau-S staining before antibody incubation and molecular mass information on detected antigens after immunoreactions added information supporting correct identification of brain tissue in the meat products. In this approach B50/growth-associated protein (B50), glial fibrillary acidic protein (GFAP), myelin basic protein (MBP), neurofilament (NF), neuron-specific enolase (NSE) and synaptophysin (Syn) proteins were detected in raw luncheon meat and a liver product enriched with brain tissue at a level of 5% (m/m). Only MBP and NSE were considered suitable biomarkers for detection of 1% (m/m) brain tissue in meat products pasteurised at 70^oC or sterilised at 115^oC. The use of an anti-monkey MBP instead of anti-human MBP enabled speciation of the CNS material whether from bovine and ovine brains or from porcine brain tissue. This immunoblot assay potentiates the analysis of approximately 70 samples within 8h, including sample preparation and the simultaneous probing of NSE and MBP target antigens.
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